Biomimetic NAD(+) models for tandem cofactor regeneration, horse liver alcohol dehydrogenase recognition of 1,4-NADH derivatives, and chiral synthesis.

The biocatalysis discipline has the potential to generate important chiral organic compounds by the use of enzymes, usually in the presence of critical co-factors. Therefore, practical methods for the regeneration of the co-enzyme, 1,4-NADH, have continued to be of significance in the biocatalysis field. In this vain, a variety of transition metal hydrides have been evaluated as catalysts for the regioselective reduction of NAD+ and NAD+ models to the corresponding 1,4-NADH derivatives in order to attempt to develop faster rates and a more economical regeneration process. In the most significant example, Steckhan and co-workers have described the use of in situ generated [Cp*Rh(bpy)(H)]+ (Cp* = η-C5Me5, bpy = 2,2'-bipyridyl), for the regiospecific reduction of natural NAD+ to 1,4-NADH, and then demonstrated the co-factor regeneration process in